Proceedings of the XLVII Italian Society of Agricultural Genetics - SIGA Annual Congress

Verona, Italy - 24/27 September, 2003

ISBN 88-900622-4-X

Poster Abstract - 5.42

ANALYSIS OF SEQUENCE POLYMORPHISMS IN CODING AND NON CODING REGIONS OF TAP2 GENE FOR THE STUDY OF INTERSPECIFIC DIFFERENTIATION IN TOMATO

A. SCIALPI, P. BOGANI, M. BUIATTI

Dipartimento di Biologia Animale e Genetica “Leo Pardi”, Università di Firenze

anionic peroxidase, Lycopersicon, sequence analysis, genetic diversity, phylogenesis

Plant peroxidases oxidize polyphenols, flavonoids and alkaloids (Gaspar et al., 1982; Blom et al., 1991) or phytoregulators such as IAA (Gazaryan et al., 1996) catalyzing the reduction of hydrogen peroxide into water, using electrons from diverse donor molecules.

Tap2 gene codes for a higly anionic peroxidase ABA-induced in suberizing tomato fruits. The gene consists of three exons and two introns and the sequence has been deposited in the GenBank (Robert and Kolattukudy, 1989).

In this work we report data obtained from the sequencing of the full-length gene in eleven species of the genus Lycopersicon with the aim to evaluate their genetic diversity and species relationships on the base of sequence level variations both in coding and non coding regions of the gene.

The primers utilized for PCR amplification were located from 329 to 348 nt the forward, and from 2525 to 2505 nt the reverse one, resulting in the synthesis of a fragment 2196 bp long, containing the three exons interrupted by two intron sequences.

Preliminary results obtained show differences in the levels of polymorphism between exons and introns determining different topologies of phylogenetic trees according to the functional units used.

Particularly our results focus on the understanding of the informative value of sequence variations depending on their localization in the gene in order to provide further informations for the study of genetic diversity and, hence, the dynamics of the genome evolution.

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Gaspar T., Penel C., Thorpe T., Greppini H. (1982). In: Peroxidases 1970-80. A survey of their biochemical and physiological roles in higher plants, University of geneva, Switzerland.

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