Proceedings of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress

Giardini Naxos, Italy - 18/21 September, 2002

ISBN 88-900622-3-1

Poster Abstract - 4.21

THE USE OF TETRA-PRIMER ARMS-PCR FOR GENOTYPING SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) IN BARLEY

CHIAPPARINO E.*, LEE D.*, TUBEROSA R.**

*) NIAB, Huntingdon Road, Cambridge CB3 0LE, UK

**) Dipartimento di Scienze e Tecnologie Agroambientali, Università di Bologna, Italy

barley, SNP, molecular markers,varieties, genotyping

Single nucleotide polymorphisms (SNPs) are the most abundant form of DNA polymorphism. Their use as molecular markers is gaining interest because they have great potential for applications in plant breeding programmes, for the identification of genetic loci affecting traits of interest, and for the characterisation and exploitation of genetic resources.

In addition, a dramatic increase in the number of DNA sequences submitted to databases is facilitating the identification of SNPs in many species, while the single most important factor preventing their widespread development and use appears to be the cost.

A simple and economical method involving a single PCR reaction is reported here for barley SNPs genotyping. Using the tetra-primers ARMS-PCR procedure (Ye et al., 2001), we have been able to unambiguously assay SNPs in a set of varieties of cultivated barley. The results show the reliability, low cost and ease of use of this technique.